MSCs can differentiate into many different cell types, as indicated by the diagram.

How are Stem Cell Injections Given?

All of our stem cell, PRP, and prolotherapy injections are given under direct ultrasound guidance.  We first numb the skin, then using ultrasound, we guide the needle to the precise location and inject the stem cells.  The ultrasound guidance insures both accurate and safe injections.

Most commonly, stem cells are not injected by themselves.  We create a healing “cocktail” containing platelet rich plasma, stem cells, and possibly other ingredients depending on the location and structure being injected.

Following injection, the area needs to be immobilized for the next 18-24 hours to allow the stem cells to “set up” and adhere to the damaged sites.  Crutches, slings, splints or braces may be used to facilitate this.  


"Dr. Frye was my last hope. We decided on PRP to both knees.  I couldn’t believe the difference the very next day!....Thanks to Dr. Frye and his team, I’m back to getting my life back, and look forward to getting better every week. "

    -Peggy C.

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Regenerative Medicine / PRP

To learn about Regenerative Medicine (PRP and Prolotherapy) click HERE.

Stem Cell Injection Therapy

The Center for Regenerative Orthopedics

Now available at The Center For Manual Medicine 

One of the most exciting recent developments in the treatment of chronic musculoskeletal conditions is the use of adult mesenchymal stem cells. (MSCs).

What are Adult Mesenchymal Stem Cells?
Stem Cells
are undifferentiated cells that have the potential to become other, more specialized types of cells.  Stem cells are broadly categorized as either “embryonic” or “adult”.  As the name implies, embryonic stem cells are derived from human fetuses, whereas adult stem cells are obtained from adults!

Currently there are over 70 proven therapies using adult stem cells, while there are none using embryonic stem cells. This is in part because scientists have had tremendous difficulty controlling how embryonic stem cells differentiate.  Adult stem cells do not suffer from this problem.

Mesenchymal stem cells, or MSCs, are multipotent stem cells that can differentiate into avariety of cell types including: osteoblasts (bone cells), chondrocytes (cartilage cells) and adipocytes (fat cells).  This has been demonstrated in ex vivo cultures and invitro or in vivo.  MSCs are obtained from the bone marrow, most commonly the back of the iliac crest (the “hip bone”).

Click HERE to see more videos from CTRMM

How Are MSC Obtained?

Harvesting MSCs for injection therapy is done right in the office.  Doctor Frye will harvest the MSCs from adipose tissue (fat) or from bone marrow.  The adipose tissue will generally be harvested from the abdomen, while the bone marrow would be harvested from the hip bone.  In either case the skin will first be numbed with Novocain.  A very small incision will then be made for the special harvesting needle to be inserted into.  In the case of adipose harvesting, a numbing fluid will be injected into the  adipose tissue, and allowed to work for 15 minutes.  Then a special harvesting needle will be inserted to draw out the adipose tissue full of MSC cells.  Only a small amount, approx. 60 cc’s or 4 table spoons will be taken.  When harvesting bone marrow the needle will pass through the cortex of the bone and slowly draw out a small amount liquid marrow.  Both procedures are painless in most cases.  


The syringe containing the MSCs from fat or bone marrow will be placed into a special centrifuge.  The fat or marrow is spun and the stem cells are highly concentrated and passed into a new syringe, from which the injection(s) are given.


The entire process takes about an hour and is minimally uncomfortable.


Call us at (785) 271-8100
The Center for Manual Medicine
5000 SW 21st ST. Topeka, KS 66604
Copyright 2017 by Center for Manual Medicine